| 产品编号 | bs-14065R |
| 英文名称 | CRNKL1 Rabbit pAb |
| 中文名称 | 细胞凋亡水解酶样蛋白1抗体 |
| 别 名 | 1200013P10Rik; 5730590A01Rik; C80326; CRN; Crn crooked neck like 1(Drosophila); HCRN; MGC55327; MGC93283; MSTP021; SYF3; CRNL1_HUMAN; Crooked neck-like protein 1; Crooked neck homolog; hCrn. |
| 研究领域 | 细胞生物 发育生物学 细胞周期蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 100 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRNKL1: 201-300/848 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] Function: The crooked neck (crn) gene of Drosophila is essential for embryogenesis. It is thought to be involved in cell cycle progression and pre mRNA splicing. Subcellular Location: Nucleus; nuclear speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm. Tissue Specificity: Widely expressed. Highly expressed in testis. Not expressed in brain and lung. Similarity: Belongs to the crooked-neck family. Contains 17 HAT repeats. SWISS: Q9BZJ0 Gene ID: 51340 Database links: Entrez Gene: 51340 Human Omim: 610952 Human SwissProt: Q5JY65 Human SwissProt: Q9BZJ0 Human Unigene: 171342 Human Unigene: 162694 Rat |
| 产品图片 |
Sample:
Siha(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- CRNKL1 (bs-14065R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kD
Observed band size: 98 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(bs-14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(bs-14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
