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Rabbit Anti-Claudin 5 antibody (bs-10296R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-10296R
英文名称 Claudin 5
中文名称 紧密连接蛋白5抗体
别    名 AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin5; Claudin-5;CLDN 5; CLDN5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; Androgen withdrawal and apoptosis induced protein RVP1 like; CLD5_HUMAN.  
研究领域 信号转导  细胞粘附分子  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 23kDa
细胞定位 细胞膜 细胞外基质 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Claudin 5:29-81/218 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

Function:
Plays a major role in tight junction-specific obliteration of the intercellular space.

Subunit:
Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ.

Subcellular Location:
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Similarity:
Belongs to the claudin family.

SWISS:
O00501

Gene ID:
7122

Database links:

Entrez Gene: 7122 Human

Entrez Gene: 12741 Mouse

Omim: 602101 Human

SwissProt: Q2HJ22 Cow

SwissProt: O00501 Human

SwissProt: O54942 Mouse

Unigene: 505337 Human

Unigene: 22768 Mouse




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Protein: lung(mouse) lysates at 45ug; Primary: Anti-Claudin 5 (bs-10296R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :23 kD
Observed band size :23kD
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (bs-10296R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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