| 产品编号 | bs-14243R |
| 英文名称 | Rabbit Anti-DEFB109 antibody |
| 中文名称 | 防御素β-109/β-defensin 109抗体 |
| 别 名 | Beta defensin 109; DB109_HUMAN; DEFB109 Beta defensin 109; Defensin beta 109. |
| 研究领域 | 细胞生物 发育生物学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 7.4kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DEFB109: 23-87/87 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Beta-defensins are small cationic peptides with broad-spectrum antimicrobial activity against a variety of enveloped viruses, fungi and bacteria. Produced in mucosal epithelia and neutrophils of several species, Beta-defensins are developmentally regulated. The family of Beta-defensin proteins share a common defensin-motif that is characterized by multiple cysteine residues and a highly conserved tertiary structure. Besides playing a significant role in host immune defense, many Beta-defensins also are involved in sperm maturation and capacitation. Beta-defensin 109 is an 87 amino acid secreted protein that is notably expressed on the ocular surface and is found in reduced levels in microbial infection, therefore suggesting that this particular Beta-defensin is unlikely to have a major antimicrobial effect. Function: DEFB109 belongs to the beta defensin family. It has antibacterial activity. Subcellular Location: Secreted Similarity: Belongs to the beta-defensin family. SWISS: Q30KR1 Gene ID: 641517 Database links: Entrez Gene: 100286963 Human Entrez Gene: 641517 Human SwissProt: Q30KR1 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DEFB109) Polyclonal Antibody, Unconjugated (bs-14243R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
