| 产品编号 | bs-10440R |
| 英文名称 | NALP6 |
| 中文名称 | 富含亮氨酸重复结构域蛋白6抗体 |
| 别 名 | CLR11.4; NACHT, leucine rich repeat and PYD containing 6; NACHT, LRR and PYD containing protein 6; NACHT, LRR and PYD domains-containing protein 6; NALP6_HUMAN; NLR family, pyrin domain containing 6; Nlrp6; Nucleotide binding oligomerization domain, leucine rich repeat and pyrin domain containing 6; PAN3; PYPAF5; PYRIN containing APAF1 like protein 5; PYRIN-containing APAF1-like protein 5. |
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Specific References (4) | bs-10440R has been referenced in 4 publications.
[IF=7.419] Cai-Mei Zheng. et al. Matrix metalloproteinase-7 promotes chronic kidney disease progression via the induction of inflammasomes and the suppression of autophagy. BIOMED PHARMACOTHER. 2022 Oct;154:113565 IHC ; Mouse.
[IF=4.527] Gao M et al. Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo (a) pyrene and lipopolysaccharide? Ecotoxicol Environ Saf. 2019 Sep 24;185:109687. IHC&IHF ; Mouse.
[IF=3.829] Wu Xingping. et al. The important role of NLRP6 inflammasome in Pasteurella multocida infection. VET RES. 2022 Dec;53(1):1-12 IF ; Mouse.
[IF=3.361] Zhang J et al. NLRP6 expressed in astrocytes aggravates neurons injury after OGD/R through activating the inflammasome and inducing pyroptosis. Int Immunopharmacol. 2020 Mar;80:106183. Co-IP ; Rat.
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| 研究领域 | 神经生物学 信号转导 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NALP6: 351-450/892 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP6, have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g., CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008] Function: May contribute to arginine-vasopressin (AVP)-mediated regulation of renal salt-water balance, glucose and lipid metabolism, apoptosis, and/or cell cycle. Isoform 1 binds arginine-vasopressin but not angiotensin II and acts as an arginine-vasopressin V2 receptor. Isoform 2 binds both arginine-vasopressin and angiotensin II and acts as an angiotensin II/vasopressin receptor. Plays a role in modulating inflammatory responses in the colon to allow recovery from intestinal epithelial damage and limit tumorigenic potential. Protects against the development of colitis by controlling the composition of the gut microbiota by preventing colonization of the colon by harmful, inflammation-inducing bacteria such as Prevotella species. May mediate activation of CASP1 via ASC, promote activation of NF-kappa-B and stimulate cAMP accumulation. Subunit: Binds to ASC with its DAPIN domain. Subcellular Location: Isoform 1: Cytoplasm. Cell membrane. Nucleus membrane. Isoform 2: Cytoplasm. Cell membrane. Note=Predominantly expressed in the cell membrane. Tissue Specificity: Highly expressed in the small and large intestine, kidney and liver. Similarity: Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 3 LRR (leucine-rich) repeats. Contains 1 NACHT domain. SWISS: P59044 Gene ID: 171389 Database links: Entrez Gene: 171389 Human Omim: 609650 Human SwissProt: P59044 Human Unigene: 352611 Human
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| 产品图片 |
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NALP6 Polyclonal Antibody, Unconjugated(bs-10440R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NALP6 Polyclonal Antibody, Unconjugated(bs-10440R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
