| 产品编号 | bs-16090R |
| 英文名称 | FITM1 |
| 中文名称 | FITM1蛋白抗体 |
| 别 名 | Fat inducing protein 1; Fat inducing transcript 1; Fat storage inducing transmembrane protein 1; Fat storage-inducing transmembrane protein 1; Fat-inducing protein 1; FIT1; FITM 1; Fitm1; FITM1_HUMAN; MGC46490. |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Dog,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FITM1: 1-100/292 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FIT1 belongs to an evolutionarily conserved family of proteins involved in fat storage (Kadereit et al., 2008 [PubMed 18160536]).[supplied by OMIM, May 2008] Function: Plays an important role in lipid droplet accumulation. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Primarily expressed in heart and skeletal muscle. Similarity: Belongs to the FIT family. SWISS: A5D6W6 Gene ID: 161247 Database links: Entrez Gene: 161247 Human Entrez Gene: 68680 Mouse Omim: 612028 Human SwissProt: A5D6W6 Human SwissProt: Q91V79 Mouse Unigene: 128060 Human Unigene: 36758 Mouse |
| 产品图片 |
Protein: 293T(human) lysate at 40ug;
Primary: rabbit Anti-FITM1 (bs-16090R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 32 kD Observed band size: 28 kD 
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
