| 产品编号 | bs-16221R |
| 英文名称 | GALNT5 |
| 中文名称 | GalNAc-T5蛋白抗体 |
| 别 名 | GALNAC T5; N acetylgalactosaminyltransferase; Polypeptide GalNAc transferase 5; polypeptide N -acetylgalactosaminyltransferase 5; pp GaNTase 5; Protein UDP acetylgalactosaminyltransferase 5; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 5; UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 5. |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 106kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GALNT5: 501-600/940 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T5 (Polypeptide N-acetylgalactosaminyltransferase 5), also known as UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5, is a 940 amino acid protein that displays enzymatic activity toward EA2 peptide substrate with weaker activity toward Muc2 or Muc 1b substrates. Its N-terminal domain is involved in substrate binding and manganese coordination, while the C-terminal domain is involved in UDP-Gal binding and catalytic reaction. EXT2 directly interacts with GalNAc-T5, suggesting that these proteins may corroborate in glycosaminoglycan synthesis. Function: GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates. Subunit: Interacts with EXT2. Does not interact with EXT1, EXTL1 or EXTL3. Subcellular Location: Cell Membrane and Golgi Apparatus Similarity: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. SWISS: Q7Z7M9 Gene ID: 11227 Database links: Entrez Gene: 11227 Human Entrez Gene: 241391 Mouse SwissProt: Q7Z7M9 Human SwissProt: Q8C102 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
