| 产品编号 | bs-16284R |
| 英文名称 | GPR107 |
| 中文名称 | G蛋白偶联受体107抗体 |
| 别 名 | bA138E2.2; DKFZp667C222; FLJ16312; FLJ20998; FLJ22591; GP107_HUMAN; G protein coupled receptor 107; GCDRP; GPR 107; KIAA1624; Lung seven transmembrane receptor 1; LUSTR1; MGC126118; MGC15440; protein GPR107; RP11 88G17. |
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Specific References (1) | bs-16284R has been referenced in 1 publications.
[IF=4.698] Mariami Jasaszwili. et al. The effects of neuronostatin on proliferation and differentiation of rat primary preadipocytes and 3T3-L1 cells. Bba-Mol Cell Biol L. 2021 Nov;1866:159018 WB ; Rat.
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| 研究领域 | 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC=1:50-200, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR107: 301-400/600 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPR107 (G protein-coupled receptor 107), also known as LUSTR1 (lung seven transmembrane receptor 1) or KIAA1624, is a 600 amino acid multi-pass membrane protein that belongs to the LU7TM family. Localizing to membrane, GPR107 exists as three alternatively spliced isoforms. Function: GPR107 is a G-protein coupled receptor which contains a carboxy-terminal seven transmembrane domain (LUSTR domain) similar to GPCRs. Three named isoforms exist. Subcellular Location: Cell membrane; integral to membrane. Similarity: Belongs to the LU7TM family. SWISS: Q5VW38 Gene ID: 57720 Database links: Entrez Gene: 57720 Human SwissProt: Q5VW38 Human Unigene: 512461 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Lane 5: Human PANC-1 cell lysates Lane 6: Human HeLa cell lysates Lane 7: Human SH-SY5Y cell lysates Lane 8: Human A549 cell lysates Lane 9: Human HUVEC cell lysates Lane 10: Human Jurkat cell lysates Primary: Anti-GPR107 (bs-16284R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 65 kDa 
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-GPR107 (bs-16284R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR107) Polyclonal Antibody, Unconjugated (bs-16284R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
