| 产品编号 | bs-16381R |
| 英文名称 | hair cortex Cytokeratin Rabbit pAb |
| 中文名称 | 毛皮质细胞角蛋白/细胞角蛋白40抗体 |
| 别 名 | CK-40; Cytokeratin-40; Cytokeratin40; K1C40_HUMAN; Cytokeratin 40; Keratin, type I cytoskeletal 40; Keratin-40; KRT40; Type I hair keratin Ka36. |
| 研究领域 | 细胞生物 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human hair cortex Cytokeratin: 151-250/431 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Keratin 40 (KRT40), also known as Cytokeratin-40 (CK-40) or KA36, is a 431 amino acid protein that belongs to the intermediate filament family. As a heterotetramer of two type I and two type II keratins, Keratin 40 may play a role in late hair differentiation. While weakly expressed in tongue, breast, colon and small intestine, Keratin 40 is highly expressed in skin and scalp. The gene that encodes Keratin 40 consists of approximately 9,420 bases and maps to human chromosome 17q21.2. Encoding more than 1,200 genes, chromosome 17 comprises over 2.5% of the human genome. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of ovary, colon, prostate gland and fallopian tubes. Function: The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. Subunit: Heterotetramer of two type I and two type II keratins. Tissue Specificity: Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level). Similarity: Belongs to the intermediate filament family. SWISS: Q6A162 Gene ID: 125115 Database links: Entrez Gene: 125115 Human Entrez Gene: 406221 Mouse SwissProt: Q6A162 Human SwissProt: Q6IFX3 Mouse Unigene: 534929 Human Unigene: 422372 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
