产品中心-北京博奥森生物技术有限公司

产品编号	bs-10011R
英文名称	Occludin
中文名称	紧密连接蛋白抗体
别名	Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.
	Specific References (62) \| bs-10011R has been referenced in 62 publications. [IF=13.352] Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579 IF ; Pig. PubMed:36265358 [IF=10.479] Shiyun Chen. et al. Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. J Adv Res. 2021 Nov;: IF ; Mouse. PubMed:10.1016/j.jare.2021.11.017 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119 WB ; Mouse. PubMed:36109005 [IF=9.229] Xiao Wang. et al. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX IHC ; Mouse. PubMed:34261306 [IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113 WB ; Mouse. PubMed:35718141 [IF=8.025] Wei Hao. et al. Ginger polysaccharides relieve ulcerative colitis via maintaining intestinal barrier integrity and gut microbiota modulation. INT J BIOL MACROMOL. 2022 Oct;219:730 IF ; Mouse. PubMed:35963344 [IF=7.963] Jun Yan. et al. Intestinal toxicity of micro- and nano-particles of foodborne titanium dioxide in juvenile mice: Disorders of gut microbiota–host co-metabolites and intestinal barrier damage. Sci Total Environ. 2022 May;821:153279 IHC ; Mouse. PubMed:35074372 [IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188 WB ; Mouse. PubMed:34875328 [IF=7.31] Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251 WB,IF ; Pig,Mouse. PubMed:36846717 [IF=7.129] Peihao Sun. et al. Lactational exposure to Deoxynivalenol causes mammary gland injury via inducing inflammatory response and impairing blood-milk barrier integrity in mice. ECOTOX ENVIRON SAFE. 2023 Apr;255:114773 IF,WB ; Mouse. PubMed:37003064 [IF=7] Zhang, Yaxin. et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. npj Science of Food. 2022 Aug;6(1):1-14 IHC ; Rat. PubMed:10.1038/s41538-022-00156-0 [IF=6.823] Caijun Zhao. et al. Aryl hydrocarbon receptor activation by Lactobacillus reuteri tryptophan metabolism alleviates Escherichia coli-induced mastitis in mice. Plos Pathog. 2021 Jul;17(7):e1009774 IHC ; Mouse. PubMed:34297785 [IF=6.656] Wei Hao. et al. Classical prescription Huanglian Decoction relieves ulcerative colitis via maintaining intestinal barrier integrity and modulating gut microbiota. PHYTOMEDICINE. 2022 Sep;:154468 IF ; Mouse. PubMed:36209702 [IF=6.59] Rongrong Fu. et al. Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.. FRONT NUTR. 2022 Dec;9:1062961-1062961 WB, IHC ; Mouse. PubMed:36590200 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965 WB ; Duck. PubMed:34775344 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242 WB ; Pig. PubMed:37108405 [IF=6.107] Xin Liu. et al. Alteration of gut microbiome and metabolome by Clostridium butyricum can repair the intestinal dysbiosis caused by antibiotics in mice. ISCIENCE. 2023 Feb;:106190 IHC ; Mouse. PubMed:36895644 [IF=6.073] Huang Li. et al. Gut microbiota decreased inflammation induced by chronic unpredictable mild stress through affecting NLRP3 inflammasome. FRONT CELL INFECT MI. 2023 May;13:558 WB ; Rat. PubMed:37293210 [IF=6.064] Irene Palenca. et al. N-Palmitoyl-D-Glucosamine Inhibits TLR-4/NLRP3 and Improves DNBS-Induced Colon Inflammation through a PPAR-α-Dependent Mechanism. BIOMOLECULES. 2022 Aug;12(8):1163 IF ; Mouse. PubMed:36009057 [IF=5.988] Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678 IHC, IF ; Rat. PubMed:35873589 [IF=5.714] Yingxue Li. et al. ProBDNF signaling is involved in periodontitis-induced depression-like behavior in mouse hippocampus. INT IMMUNOPHARMACOL. 2023 Mar;116:109767 WB,IF ; Mouse. PubMed:36738676 [IF=5.714] Mingwei Tong. et al. The Trichinella spiralis-derived antigens alleviate HFD-induced obesity and inflammation in mice. INT IMMUNOPHARMACOL. 2023 Apr;117:109924 IHC ; Mouse. PubMed:36848791 [IF=5.572] Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc−/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730 WB ; Mouse. PubMed:36925038 [IF=5.546] Yaqi Chang. et al. Glucagon‐like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032 IHC ; Pig. PubMed:32960454 [IF=5.265] Li, Ke. et al. The Prevention Effect of Lactobacillus plantarum 17–5 on Escherichia coli-Induced Mastitis in Mice. PROBIOTICS ANTIMICRO. 2023 Feb;:1-9 WB ; Mouse. PubMed:36790662 [IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640 WB ; Rat. PubMed:37196812 [IF=4.546] Kexin Wang. et al. Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury. TOXINS. 2022 May;14(5):337 WB ; Bovine. PubMed:35622584 [IF=4.546] Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816 IHC ; Mouse. PubMed:33801268 [IF=4.546] Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863 WB ; Pig. PubMed:34941701 [IF=4.432] Juan Zhang. et al. Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora. Eur J Pharmacol. 2022 Feb;916:174730 IHC ; Mouse. PubMed:34968462
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Rat,Mouse,Human (predicted: Sheep,Cow,Pig,Dog)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	59kDa
细胞定位	细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Occludin: 151-250/522 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]. Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Subunit: Interacts with TJP1/ZO1 and with VAPA. Subcellular Location: Membrane; Multi-pass membrane protein. Cell junction, tight junction. Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts. Similarity: Belongs to the ELL/occludin family. Contains 1 MARVEL domain. SWISS: Q16625 Gene ID: 100506658 Database links: Entrez Gene: 18260 Mouse Entrez Gene: 100506658 Human Entrez Gene: 397236 Pig Entrez Gene: 83497 Rat Entrez Gene: 403844 Dog Omim: 602876 Human SwissProt: Q28269 Dog SwissProt: Q16625 Human SwissProt: Q61146 Mouse SwissProt: Q6P6T5 Rat Unigene: 592605 Human Unigene: 4807 Mouse Unigene: 31429 Rat
产品图片	Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Occludin (bs-10011R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 60 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Occludin (bs-10011R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Sample: Testis (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-Occludin (bs-10011R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: Hela (Human) Cell Lysate at 30 ug Primary: Anti-Occludin (bs-10011R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65/55/38/28 kD Observed band size: 62/52/40/25 kD Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (bs-10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记