产品中心-北京博奥森生物技术有限公司

产品编号	bs-16698R
英文名称	phospho-IRE1 (Ser724)
中文名称	磷酸化IRE1蛋白抗体
别名	IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1.
	Specific References (12) \| bs-16698R has been referenced in 12 publications. [IF=7.675] Reziyamu Wufuer. et al. Distinct Roles of Nrf1 and Nrf2 in Monitoring the Reductive Stress Response to Dithiothreitol (DTT). ANTIOXIDANTS-BASEL. 2022 Aug;11(8):1535 WB ; Human. PubMed:36009254 [IF=7.129] Gang Yu. et al. The ZIP9-centered androgen pathway compensates for the 2605 MHz radiofrequency electromagnetic radiation-mediated reduction in resistance to H2O2 damage in Sertoli cells of adult rats. ECOTOX ENVIRON SAFE. 2023 Apr;254:114733 WB ; Rat. PubMed:36889209 [IF=6.706] Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994 WB ; Rat. PubMed:36501024 [IF=6.543] Fan Kexia. et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev. 2022;2022:5652586 WB ; Mouse. PubMed:35368865 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242 WB ; Pig. PubMed:37108405 [IF=6.023] Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697 WB ; Rat. PubMed:34826549 [IF=5.878] Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. 2021 Dec 02 WB ; Mouse ,Human. PubMed:34859513 [IF=5.396] Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 Feb;: WB ; Rat. PubMed:35262539 [IF=4.868] Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. WB ; Mouse. PubMed:30728884 [IF=4.52] Zhu YP et al. Unification of Opposites between Two Antioxidant Transcription Factors Nrf1 and Nrf2 in Mediating Distinct Cellular Responses to the Endoplasmic Reticulum Stressor Tunicamycin. Antioxidants (Basel). 2019 Dec 19;9(1). pii: E4. WB ; Human. PubMed:31861550 [IF=4.294] Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187 WB ; Human. PubMed:34813867 [IF=3.038] Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res . 2020 Oct;45(10):2302-2311. WB ; rat. PubMed:32681444
研究领域	细胞生物信号转导激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Rat,Mouse,Human (predicted: Sheep,Horse,Cow,Pig)
产品应用	WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	107kDa
细胞定位	细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724: RH(p-S)FS
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway. Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed. High levels observed in pancreatic tissue. Post-translational modifications: Autophosphorylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain. SWISS: O75460 Gene ID: 2081 Database links: Entrez Gene: 10595 Human Entrez Gene: 2081 Human Entrez Gene: 78943 Mouse Entrez Gene: 498013 Rat Omim: 604033 Human SwissProt: O75460 Human SwissProt: Q76MJ5 Human SwissProt: Q9EQY0 Mouse SwissProt: Q9Z2E3 Mouse Unigene: 133982 Human Unigene: 592041 Human Unigene: 700027 Human Unigene: 20452 Mouse Unigene: 340943 Mouse Unigene: 226435 Rat
产品图片	Sample: Lane 1: Human 293T cell lysates Lane 2: Human DU145 cell lysates Lane 3: Human HeLa cell lysates Lane 4: Human Jurkat cell lysates Primary: Anti-phospho-IRE1 (Ser724) (bs-16698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kDa Observed band size: 130 kDa

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记