产品编号 | bs-16708R |
英文名称 | TRAX Rabbit pAb |
中文名称 | 转位蛋白相关X因子抗体 |
别 名 | Translin associated factor X; Translin-associated factor X; Translin-associated protein X; TSNAX; TSNAX_HUMAN. |
研究领域 | 细胞生物 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 33 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TRAX: 21-120/290 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a protein which specifically interacts with translin, a DNA-binding protein that binds consensus sequences at breakpoint junctions of chromosomal translocations. The encoded protein contains bipartite nuclear targeting sequences that may provide nuclear transport for translin, which lacks any nuclear targeting motifs. [provided by RefSeq, Jul 2008] Function: Possible role in spermatogenesis. Subcellular Location: Cytoplasm > perinuclear region. Nucleus. Expressed in the cytoplasm in the presence of TSN. Post-translational modifications: Sumoylated. Similarity: Belongs to the translin family. SWISS: Q99598 Gene ID: 7257 Database links: Entrez Gene: 7257 Human Omim: 602964 Human SwissProt: Q99598 Human Unigene: 13318 Human |
产品图片 |
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-TRAX (bs-16708R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAX) Polyclonal Antibody, Unconjugated (bs-16708R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |