| 产品编号 | bs-16751R |
| 英文名称 | KLC3 Rabbit pAb |
| 中文名称 | 驱动蛋白轻链3抗体 |
| 别 名 | Kinesin light chain 2; Kinesin light chain 3; KLC2; KLC2 like; KLC2-like; KLC2L; KLC3; KLC3_HUMAN; KLCt; KNS2B. |
| 研究领域 | 细胞生物 发育生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Cow,Horse, Orangutan) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLC3: 401-504/504 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two kinesin light chain (KLC) molecules. KLCs are thought to typically be involved in binding cargo and regulating kinesin activity. In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural components of sperm tails and mitochondria. [provided by RefSeq, Jul 2008] Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Subcellular Location: Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria. Similarity: Belongs to the kinesin light chain family. Contains 5 TPR repeats. SWISS: Q6P597 Gene ID: 147700 Database links: Entrez Gene: 147700 Human Entrez Gene: 232943 Mouse Entrez Gene: 100173098 Orangutan Omim: 601334 Human SwissProt: Q6P597 Human SwissProt: Q91W40 Mouse SwissProt: Q5R8E2 Orangutan Unigene: 298079 Human Unigene: 213403 Mouse Unigene: 161815 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLC3) Polyclonal Antibody, Unconjugated (bs-16751R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
