产品编号 | bs-16797R |
英文名称 | KMT4 |
中文名称 | 组蛋白H3 K79甲基转移酶抗体 |
别 名 | Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase (S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like protein; DOT1, S. cerevisiae, homolog of; DOT1L; EC 2.1.1.43; H3 K79 HMTase; Histone H3 K79 methyltransferase; Histone H3 methyltransferase; Histone H3 methyltransferase DOT1-like; Histone lysine N methyltransferase H3 lysine 79 specific; Histone methyltransferase DOT1L; KIAA1814; DOT1L_HUMAN; KMT4; Lysine N methyltransferase 4. |
研究领域 | 免疫学 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Cow,Sheep,African Green Monkey) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 185kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human KMT4: 51-150/1739 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] Function: The KMT4 / Dot1 (disruptor of telomeric silencing) family are a class of histone methyltransferases that play an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications. Subunit: Interacts with MLLT10. Subcellular Location: Nuclear Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. Contains 1 DOT1 domain. SWISS: Q8TEK3 Gene ID: 84444 Database links: Entrez Gene: 84444 Human Omim: 607375 Human SwissProt: Q8TEK3 Human Unigene: 713641 Human Unigene: 228827 Rat |
产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- KMT4 (bs-16797R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 185 kD Observed band size: 170 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |