| 产品编号 | bs-16803R |
| 英文名称 | KPNA3 |
| 中文名称 | KPNA3蛋白抗体 |
| 别 名 | SRP1 gamma; hSRP1; IMA3_HUMAN; Importin alpha 3; Importin alpha 3 subunit; Importin alpha 4; Importin alpha Q2; Importin subunit alpha-3; IPOA4; Karyopherin alpha 3; Karyopherin alpha 3 importin alpha 4; Karyopherin alpha 3 subunit; Karyopherin subunit alpha 3; Karyopherin subunit alpha-3; KPNA 3; Kpna3; Qip2; SRP1; SRP1 gamma; SRP1-gamma; SRP1gamma; SRP4. |
| 研究领域 | 细胞生物 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rhesus monkey) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KPNA3: 1-100/521 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009] Function: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Ubiquitous. Highest levels in heart and skeletal muscle. Similarity: Belongs to the importin alpha family. Contains 10 ARM repeats. Contains 1 IBB domain. SWISS: O00505 Gene ID: 3839 Database links: Entrez Gene: 3839 Human Entrez Gene: 418870 Chicken Entrez Gene: 16648 Mouse Entrez Gene: 706925 Rhesus monkey Omim: 601892 Human SwissProt: O00505 Human SwissProt: O35344 Mouse Unigene: 527919 Human Unigene: 25548 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
