| 产品编号 | bs-16806R |
| 英文名称 | KPNA6 |
| 中文名称 | KPNA6蛋白抗体 |
| 别 名 | FLJ11249; IMA7_HUMAN; Importin alpha 7 subunit; Importin alpha S2; Importin subunit alpha 7; Importin subunit alpha-7; IPOA 7; IPOA7; Karyopherin alpha 6; Karyopherin alpha 6 (importin alpha 7); Karyopherin subunit alpha 6; Karyopherin subunit alpha-6; KPNA 6; KPNA 7; Kpna6; KPNA7; MGC17918; OTTHUMP00000004532. |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Pig,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KPNA6: 101-200/536 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008] Function: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Subunit: Interacts with ZIC3 (By similarity). Forms a complex with importin subunit beta-1. Tissue Specificity: Widely expressed. Similarity: Belongs to the importin alpha family. Contains 10 ARM repeats. Contains 1 IBB domain. SWISS: O60684 Gene ID: 23633 Database links: Entrez Gene: 23633 Human Entrez Gene: 16650 Mouse Omim: 610563 Human SwissProt: O60684 Human SwissProt: O35345 Mouse Unigene: 470588 Human Unigene: 711019 Human Unigene: 202358 Mouse Unigene: 139167 Rat |
| 产品图片 |
Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-KPNA6 (bs-16806R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 63 kD 
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-KPNA6 (bs-16806R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD 
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-KPNA6 antibody (bs-16806R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
