| 产品编号 | bs-17082R |
| 英文名称 | KAZALD1 |
| 中文名称 | KAZALD1蛋白抗体 |
| 别 名 | Kazal-type serine protease inhibitor domain-containing protein 1; KAZALD1; KAZD1_HUMAN. |
| 研究领域 | 细胞生物 发育生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KAZALD1: 71-170/304 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
BONO1, also known as KAZALD1 (kazal-type serine protease inhibitor domain-containing protein 1), IGFBP-rP10, FKSG28 or FKSG40, is a 304 amino acid secreted extracellular matrix protein that promotes matrix assembly. BONO1 is expressed in developing bones and odontoblasts in teeth, where it plays a role in osteoblast proliferation during bone formation and regeneration. BONO1 is also expressed at high levels in spleen, and is found at lower levels in lung, skin, urinary bladder, brain, tongue, kidney and large intestine. Existing as two alternatively spliced isoforms, BONO1 contains one Kazal-like domain, an IGFBP N-terminal domain and a single Ig-like C2-type (immunoglobulin-like) domain. The gene encoding BONO1 maps to human chromosome 10, which contains over 800 genes and 135 million nucleotides. Cockayne syndrome, Cockayne syndrome and trisomy 10 are associated with defects in chromosome 10. Function: Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly. Subcellular Location: Secreted > extracellular space > extracellular matrix. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. SWISS: Q96I82 Gene ID: 81621 Database links: Entrez Gene: 81621 Human SwissProt: Q96I82 Human Unigene: 534859 Human |
| 产品图片 |
Sample:
DU145(Human) Cell Lysate at 40 ug Primary: Anti-KAZALD1 (bs-17082R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD 
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (bs-17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (bs-17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
