| 产品编号 | bs-17145R |
| 英文名称 | TRMT5 Rabbit pAb |
| 中文名称 | TRMT5蛋白抗体 |
| 别 名 | KIAA1393; M1G methyltransferase; M1G-methyltransferase; TRM5; TRM5 tRNA methyltransferase 5 homolog(S. cerevisiae); TRMT5; TRMT5_HUMAN; tRNA(guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5; tRNA N1G37 methyltransf |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT5: 201-300/509 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM, Mar 2008] Function: Specifically methylates guanosine-37 in various tRNAs. Not dependent on the nature of the nucleoside 5' of the target nucleoside. Subunit: Monomer. Subcellular Location: Cytoplasm. Similarity: Belongs to the TRM5 / TYW2 family. SWISS: Q32P41 Gene ID: 57570 Database links: Entrez Gene: 57570 Human Entrez Gene: 76357 Mouse Omim: 611023 Human SwissProt: Q32P41 Human SwissProt: Q9D0C4 Mouse Unigene: 380159 Human Unigene: 311344 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
