产品编号 | bs-10557R |
英文名称 | GOT2 |
中文名称 | 谷草转氨酶2抗体 |
别 名 | ASPATA; FABP pm; Got 2; Aspartate aminotransferase; Aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; EC 2.6.1.1; FABP 1; FABPpm; Fatty acid binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; Glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase, mitochondrial; Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) ; KAT4; KATIV; Kynurenine aminotransferase IV; mAspAT; mitAAT; Mitochondrial aspartate aminotransferase; OTTMUSP00000017748; Plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A; MGC102129; MGC115763; AL022787; AATM_HUMAN. |
研究领域 | 心血管 细胞生物 细胞凋亡 转录调节因子 激酶和磷酸酶 线粒体 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse,Human (predicted: Rabbit,Rat) |
产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 45kDa |
细胞定位 | 细胞浆 细胞膜 线粒体 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GOT2: 81-180/430 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower. Function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Subunit: Homodimer. Subcellular Location: Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane. Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. SWISS: P00505 Gene ID: 2806 Database links: Entrez Gene: 2806 Human Entrez Gene: 14719 Mouse Omim: 138150 Human SwissProt: P00505 Human SwissProt: P05202 Mouse Unigene: 599470 Human Unigene: 230169 Mouse Unigene: 98650 Rat |
产品图片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug Primary: Anti-GOT2 (bs-10557R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |