| 产品编号 | bs-17207R |
| 英文名称 | Josephin-2 Rabbit pAb |
| 中文名称 | JOSD2蛋白抗体 |
| 别 名 | FLJ29018; JOS2_HUMAN; JOSD 2; JOSD2; Josephin 2; Josephin domain containing 2; Josephin domain containing protein 2; Josephin domain-containing protein 2; Josephin-2; Josephin2; SBBI54. |
| 研究领域 | 细胞生物 免疫学 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Josephin-2: 91-188/188 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Josephin-2, also known as JOSD2 (josephin domain containing 2), is a 188 amino acid protein that contains one josephin domain and is encoded by a gene that maps to human chromosome 19. Consisting of around 63 million bases with over 1,400 genes, chromosome 19 makes up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family, and Fc?receptors. Key genes for eye color and hair color also map to chromosome 19. Peutz-Jeghers syndrome, spinocerebellar ataxia type 6, the stroke disorder CADASIL, hypercholesterolemia and insulin-dependent diabetes have been linked to chromosome 19. Translocations with chromosome 19 and chromosome 14 can be seen in some lymphoproliferative disorders and typically involve the proto-oncogene BCL3. Function: Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme. Similarity: Contains 1 Josephin domain. SWISS: Q8TAC2 Gene ID: 126119 Database links: Entrez Gene: 126119 Human Entrez Gene: 66124 Mouse SwissProt: Q8TAC2 Human SwissProt: Q9CR30 Mouse Unigene: 467151 Human Unigene: 358691 Mouse Unigene: 22861 Rat |
| 产品图片 |
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human HT-1080 cell lysates
Lane 4: Human A431 cell lysates
Primary: Anti-Josephin-2 (bs-17207R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 15,21 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
