| 产品编号 | bs-17285R |
| 英文名称 | Schlafen 8 Rabbit pAb |
| 中文名称 | Schlafen 8蛋白抗体 |
| 别 名 | Slfn8; SLFN8_MOUSE; Schlafen family member 8; mSLFN8; Slfn8 pending. |
| 研究领域 | 细胞生物 t-淋巴细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse |
| 产品应用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 104 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Schlafen 8: 831-910/910 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The regulatory networks governing development and differentiation of hematopoietic cells are incompletely understood. Members of the Schlafen protein family have been implicated in the regulation of cell growth and T cell development. Four new members, Schlafen 5, Schlafen 8, Schlafen 9 and Schlafen 10, which belong to a distinct subgroup within this gene family have been identified and chromosomally mapped. The characteristic feature of these proteins is the presence of sequence motifs identifying them as distinct members of the superfamily I of DNA/RNA helicases. SWISS: B1ARD8 Gene ID: 276950 Database links: Entrez Gene: 276950 Mouse SwissProt: B1ARD8 Mouse |
| 产品图片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Schlafen 8 antibody (bs-17285R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
