| 产品编号 | bs-17339R |
| 英文名称 | hnRNP A0 Rabbit pAb |
| 中文名称 | 异质核糖核蛋白A0抗体 |
| 别 名 | HNRNPA0; Heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0; HNRNPA0; ROA0_HUMAN. |
| 研究领域 | 细胞生物 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog, Cynomolgus Monkey,Rhesus monkey,Gorilla,Marmoset (common)) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human hnRNP A0: 1-100/305 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB), and its homolog hnRNP L, bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of sev-eral viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a protein that is localized in the nucleoplasm. hnRNP L, like hnRNP I, is also localized in the nucleoplasm. Function: This protein is a component of ribonucleosomes. Subcellular Location: Nucleus. Component of ribonucleosomes. Post-translational modifications: Arg-291 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Contains 2 RRM (RNA recognition motif) domains. SWISS: Q13151 Gene ID: 10949 Database links: Entrez Gene: 416302 Chicken Entrez Gene: 745876 Chimpanzee Entrez Gene: 10949 Human Entrez Gene: 77134 Mouse Omim: 609409 Human SwissProt: Q13151 Human SwissProt: Q9CX86 Mouse Unigene: 645902 Human Unigene: 96996 Human Unigene: 490274 Mouse |
| 产品图片 |
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-hnRNP A0 (bs-17339R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
