产品编号 | bs-10610R |
英文名称 | DRD1 |
中文名称 | 多巴胺受体D1抗体 |
别 名 | dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1A; DRD1_HUMAN; DRD 1A. |
Specific References (1) | bs-10610R has been referenced in 1 publications.
[IF=3.322] Hua-chun Miao. et al. Expression changes of c-Fos and D1R/p-ERK1/2 signal pathways in nucleus accumbens of rats after ketamine abuse. BIOCHEM BIOPH RES CO. 2022 Sep;: IHC, WB ; Rat.
|
|
研究领域 | 肿瘤 免疫学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 50kDa |
检测分子量 | 65-80 kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Dopamine Receptor D1: 11-100/446 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008] Function: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21728 Gene ID: 1812 Database links: Entrez Gene: 1812 Human Entrez Gene: 13488 Mouse Omim: 126449 Human SwissProt: P21728 Human SwissProt: Q61616 Mouse Unigene: 2624 Human Unigene: 54161 Mouse Unigene: 24039 Rat |
产品图片 |
Sample:A549 Cell(Human)Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD
Sample:
A431 Cell(Human)Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |