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Rabbit Anti-DRD1  antibody (bs-10610R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-10610R
英文名称 DRD1
中文名称 多巴胺受体D1抗体
别    名 dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1A; DRD1_HUMAN; DRD 1A.  
Specific References  (1)     |     bs-10610R has been referenced in 1 publications.
[IF=3.322] Hua-chun Miao. et al. Expression changes of c-Fos and D1R/p-ERK1/2 signal pathways in nucleus accumbens of rats after ketamine abuse. BIOCHEM BIOPH RES CO. 2022 Sep;:  IHC, WB ;  Rat.  
研究领域 肿瘤  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 50kDa
检测分子量 65-80 kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Dopamine Receptor D1: 11-100/446 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Function:
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity:
Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P21728

Gene ID:
1812

Database links:

Entrez Gene: 1812 Human

Entrez Gene: 13488 Mouse

Entrez Gene: 24316 Rat

Omim: 126449 Human

SwissProt: P21728 Human

SwissProt: Q61616 Mouse

SwissProt: P18901 Rat

Unigene: 2624 Human

Unigene: 54161 Mouse

Unigene: 24039 Rat



产品图片
Sample:A549 Cell(Human)Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Sample:
A431 Cell(Human)Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell: SH-SY5Y
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10610R) on SH-SY5Y(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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