| 产品编号 | bs-17387R |
| 英文名称 | HRC Rabbit pAb |
| 中文名称 | HRC蛋白抗体 |
| 别 名 | HCP; HRC; HRC histidine rich calcium binding protein; MGC133236; Sarcoplasmic reticulum histidine-rich calcium-binding protein; SRCH_HUMAN. |
| 研究领域 | 细胞生物 免疫学 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 77 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HRC: 21-120/699 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HRC is a 699 amino acid that binds low-density lipoprotein with high affinity. As a high capacity calcium binding protein, HRC regulates the sequestration and release of calcium in the lumen of the junctional sarcoplasmic reticulum (SR) of cardiac, skeletal and smooth muscle. This mechanism may involve direct interaction between HRC and the cytoplasmic domain of Triadin, an integral membrane protein of the SR. The gene encoding HRC maps to human chromosome 19q13.33 and mouse chromosome 7 B4 Function: May play a role in the regulation of calcium sequestration or release in the SR of skeletal and cardiac muscle. Subcellular Location: Sarcoplasmic reticulum lumen. Similarity: To rabbit HRC. SWISS: P23327 Gene ID: 3270 Database links: Entrez Gene: 3270 Human Omim: 142705 Human SwissProt: P23327 Human Unigene: 436885 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRC) Polyclonal Antibody, Unconjugated (bs-17387R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
