| 产品编号 | bs-17574R |
| 英文名称 | OXA1L |
| 中文名称 | OXA1L蛋白抗体 |
| 别 名 | HSA; Mitochondrial inner membrane protein OXA1L; OXA1; OXA1 like protein; OXA1-like protein; OXA1Hs; Oxa1l; OXA1L_HUMAN; Oxidase (cytochrome c) assembly 1 like; Oxidase assembly 1 like protein; Oxidase assembly 1-like protein. |
| 研究领域 | 细胞生物 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OXA1L: 301-400/435 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase. Required for the correct biogenesis of ATP synthase and complex I in mitochondria. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the OXA1/ALB3/YidC family. SWISS: Q15070 Gene ID: 5018 Database links: Entrez Gene: 100719154 Guinea pig Entrez Gene: 100056354 Horse Entrez Gene: 5018 Human Entrez Gene: 69089 Mouse Entrez Gene: 562963 Zebrafish Omim: 601066 Human SwissProt: Q15070 Human SwissProt: Q8BGA9 Mouse Unigene: 151134 Human Unigene: 182340 Mouse |
| 产品图片 |
Sample:
Hcclm3(Human) Cell Lysate at 30 ug Primary: Anti-OXA1L (bs-17574R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD 
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OXA1L) Polyclonal Antibody, Unconjugated (bs-17574R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
