| 产品编号 | bs-17625R |
| 英文名称 | SPATA20 |
| 中文名称 | 精子发生相关蛋白20抗体 |
| 别 名 | SPATA20; Sperm protein SSP411; Sperm-specific protein 411; Spermatogenesis associated 20; Spermatogenesis-associated protein 20; SPT20_HUMAN; Ssp411; Tisp78; Transcript increased in spermiogenesis 78. |
| 研究领域 | 细胞生物 发育生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep,GuineaPig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPATA20: 431-530/786 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SPATA20 is a 786 amino acid protein that may be involved in fertility regulation. SPATA20 is expressed in testis in an age dependent manner and localizes to round and elongated spermatids. SPATA20 contains a conserved thioredoxin-like domain near the N-terminus region and exists as four isoforms produced by alternative splicing events. SPATA20 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. Function: May play a role in fertility regulation. Subcellular Location: Secreted. SWISS: Q8TB22 Gene ID: 64847 Database links: Entrez Gene: 100714104 Guinea pig Entrez Gene: 100069987 Horse Entrez Gene: 64847 Human Entrez Gene: 217116 Mouse Omim: 613939 Human SwissProt: Q8TB22 Human SwissProt: Q80YT5 Mouse Unigene: 103147 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
