产品编号 | bs-17635R |
英文名称 | SPATA9 Rabbit pAb |
中文名称 | 精子发生相关蛋白9抗体 |
别 名 | FLJ35906; NYD SP16; spermatogenesis associated 9; Spermatogenesis associated protein 9; Testis development protein NYD SP16. |
研究领域 | 细胞生物 免疫学 发育生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow) |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 29 kDa |
检测分子量 | |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SPATA9: 121-200/254 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
SPATA9 is a 254 amino acid single-pass membrane protein that is highly expressed in testis and pancreas with low expression in heart, lung, and brain. A component of the sperm acrosome, SPATA9 may participate in sperm capacitation and acrosome reaction, and is therefore necessary for fertilization. SPATA9 is also suggested to be involved in testicular development/spermatogenesis and may be an important factor in male infertility. No expression of SPATA9 was found in patients affected by Sertoli-cell-only syndrome, also known as Del Castillo syndrome or germ cell aplasia, which is characterized by male sterility without sexual abnormality. SPATA9 is encoded by a gene located on human chromosome 5, which consists of about 181 million base pairs, encodes around 1,000 genes and represents about 6% of human genomic DNA. Function: SPATA9 may play a role in testicular development/spermatogenesis and may be an important factor in male infertility. Three named isoforms exist. Subcellular Location: Membrane; Single-pass membrane protein SWISS: Q9BWV2 Gene ID: 83890 Database links: Entrez Gene: 83890 Human Omim: 608039 Human SwissProt: Q9BWV2 Human |
产品图片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-SPATA9 (bs-17635R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 19 kD
Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-SPATA9 (bs-17635R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 19 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |