| 产品编号 | bs-17651R |
| 英文名称 | SPEF1 Rabbit pAb |
| 中文名称 | 精子鞭毛蛋白1抗体 |
| 别 名 | C20orf28; Calponin homology and microtubule associated protein; CLAMP; SPEF1A; Sperm flagellar 1; Sperm flagellar protein 1. |
| 研究领域 | 细胞生物 免疫学 发育生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPEF1: 181-236/236 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CLAMP is a 236 amino acid protein that is present in epididymal sperm and localizes to the cell projection, as well as to the cytoplasm. Expressed in lung, brain and testis, CLAMP functions as a microtubule-associated protein that is thought to play a role in microtubule bundling. Human CLAMP exists as two alternatively spliced isoforms and shares a high degree of homology with its mouse counterpart, suggesting a conserved role between species. The gene encoding CLAMP maps to human chromosome 20, which houses over 600 genes and comprises nearly 2% of the human genome. Subcellular Location: Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia. SWISS: Q9Y4P9 Gene ID: 25876 Database links: Entrez Gene: 25876 Human Omim: 610674 Human SwissProt: Q9Y4P9 Human Unigene: 72620 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPEF1) Polyclonal Antibody, Unconjugated (bs-17651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPEF1) Polyclonal Antibody, Unconjugated (bs-17651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
