产品编号 | bs-18114R |
英文名称 | HYLS1 Rabbit pAb |
中文名称 | Hydrolethalus综合征蛋白1抗体 |
别 名 | HLS; Hydrolethalus syndrome protein 1; HYLS1; HYLS1_HUMAN. |
研究领域 | 细胞生物 发育生物学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Cow) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 34 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HYLS1: 51-150/299 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a protein localized to the cytoplasm. Mutations in this gene are associated with hydrolethalus syndrome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008] Subcellular Location: Cytoplasm. DISEASE: Defects in HYLS1 are the cause of hydrolethalus syndrome type 1 (HLS1) [MIM:236680]. HLS1 is a lethal malformation syndrome leading to stillbirth or death shortly after birth. It is characterized by hydrocephaly with absent upper midline structures of the brain, micrognathia and polydactyly. Similarity: Belongs to the HYLS1 family. SWISS: Q96M11 Gene ID: 219844 Database links: Entrez Gene: 219844 Human Omim: 610693 Human SwissProt: Q96M11 Human Unigene: 585071 Human |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HYLS1) Polyclonal Antibody, Unconjugated (bs-18114R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HYLS1) Polyclonal Antibody, Unconjugated (bs-18114R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |