产品编号 | bs-18119R |
英文名称 | ICEBERG |
中文名称 | ICEBERG蛋白抗体 |
别 名 | CAR18_HUMAN; CARD18; Caspase 1 inhibitor Iceberg; Caspase recruitment domain family member 18; Caspase recruitment domain-containing protein 18; Caspase-1 inhibitor Iceberg; ICEBERG caspase 1 inhibitor; Pseudo ICE; UNQ5804; UNQ5804/PRO19611. |
研究领域 | 细胞生物 细胞凋亡 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 10kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ICEBERG: 31-80/90 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
ICEBERG is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. Primarily expressed in the heart and placenta, ICEBERG inhibits the generation of IL-1 β which blocks apoptosis during an inflammatory stimulus. ICEBERG inhibits IL-1 beta by associating with caspase-1, thereby preventing caspase-1 from interacting with the pro-apoptotic protein RIP2. The association of ICEBERG with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of ICEBERG. The association of caspase-1 with RIP2 will inhibit apoptosis by generating IL-1 beta. However, the association of caspase-1, which serves both pro and antiapoptotic roles, with ICEBERG induces apoptosis by inhibiting the generation of IL-1 β. Function: Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2. Down-regulates the release of IL1B. Subunit: Interacts with pro-CASP1. Interacts with CARD8. Tissue Specificity: Primarily expressed in the heart and placenta. Similarity: Contains 1 CARD domain. SWISS: P57730 Gene ID: 59082 Database links: Entrez Gene: 59082 Human Omim: 605354 Human SwissProt: P57730 Human Unigene: 56279 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |