产品编号 | bs-18286R |
英文名称 | LINS |
中文名称 | LINS蛋白抗体 |
别 名 | LINES_HUMAN; LINS; Protein Lines homolog 1; Protein Lines homolog; Wnt-signaling molecule Lines homolog 1. |
研究领域 | 细胞生物 免疫学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 86kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LINS: 121-220/757 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. [provided by RefSeq, Sep 2010] Tissue Specificity: Expresssed in adult testis, prostate, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain. Similarity: Belongs to the protein lines family. SWISS: Q8NG48 Gene ID: 55180 Database links: Entrez Gene: 55180 Human Omim: 610350 Human SwissProt: Q8NG48 Human Unigene: 105633 Human |
产品图片 |
Sample:
U87MG(Human) Cell Lysate at 40 ug Primary: Anti-LINS (bs-18286R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD
Sample:
U251(Human) Cell Lysate at 40 ug Primary: Anti-LINS (bs-18286R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LINS) Polyclonal Antibody, Unconjugated (bs-18286R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |