产品中心-北京博奥森生物技术有限公司

产品编号	bs-10966R
英文名称	Actin
中文名称	肌动蛋白α1抗体
别名	muscle Actin; ACTS_HUMAN; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; Beta cytoskeletal actin; nemaline myopathy type 3. α-skeletal muscle actin; α Skeletal Muscle Actin.
	Specific References (36) \| bs-10966R has been referenced in 36 publications. [IF=19.16] Wang Huan. et al. CTC1 OB-B interaction with TPP1 terminates telomerase and prevents telomere overextension. NUCLEIC ACIDS RES. 2023 Apr;: WB ; Human. PubMed:37021555 [IF=18.187] Gong, Hui. et al. miR-146a impedes the anti-aging effect of AMPK via NAMPT suppression and NAD+/SIRT inactivation. Signal Transduct Tar. 2022 Mar;7(1):1-13 WB ; Mouse. PubMed:35241643 [IF=14.903] Chang Xiao. et al. Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy. ACTA PHARM SIN B. 2023 May;: WB ; Mouse. PubMed:10.1016/j.apsb.2023.05.019 [IF=10.435] Shi, Yesi. et al. Biomimetic nanoparticles blocking autophagy for enhanced chemotherapy and metastasis inhibition via reversing focal adhesion disassembly. J Nanobiotechnol. 2021 Dec;19(1):1-17 WB ; Human. PubMed:34952594 [IF=6.953] Meng Zhang. et al. Moutan Cortex polysaccharide ameliorates diabetic kidney disease via modulating gut microbiota dynamically in rats. Int J Biol Macromol. 2022 May;206:849 WB ; Rat. PubMed:35307460 [IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453 WB ; Human. PubMed:35339497 [IF=6.543] Huang Jian. et al. Icariside II Attenuates Methamphetamine-Induced Neurotoxicity and Behavioral Impairments via Activating the Keap1-Nrf2 Pathway. Oxid Med Cell Longev. 2022;2022:8400876 WB ; Mouse. PubMed:35387263 [IF=5.895] Ying-Cai Zhao. et al. N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:37098125 [IF=5.858] Weitong Xu. et al. Pan-mTOR inhibitors sensitize the senolytic activity of navitoclax via mTORC2 inhibition-mediated apoptotic signaling. BIOCHEM PHARMACOL. 2022 Jun;200:115045 WB ; Human. PubMed:35439535 [IF=5.834] Xue-sheng Pan. et al. Kupffer cell pyroptosis mediated by METTL3 contributes to the progression of alcoholic steatohepatitis. FASEB J. 2023 May;37(6):e22965 WB ; Mouse. PubMed:37171272 [IF=5.717] Yanxiong Huo. et al. Bifidobacterium animalis subsp. lactis A6 Enhances Fatty Acid β-Oxidation of Adipose Tissue to Ameliorate the Development of Obesity in Mice. Nutrients. 2022 Jan;14(3):598 WB ; Mouse. PubMed:10.3390/nu14030598 [IF=5.717] Antonia Giacco. et al. Mild Endurance Exercise during Fasting Increases Gastrocnemius Muscle and Prefrontal Cortex Thyroid Hormone Levels through Differential BHB and BCAA-Mediated BDNF-mTOR Signaling in Rats. Nutrients. 2022 Jan;14(6):1166 WB ; Rat. PubMed:35334826 [IF=5.572] Shaohua Hou. et al. Design, synthesis and biological evaluation of 1H-indazole derivatives as novel ASK1 inhibitors. Eur J Med Chem. 2021 Aug;220:113482 WB ; Human. PubMed:33906048 [IF=5.293] Shi C. et al. Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice With Echinococcus granulosus at Different Infection Stages and Their Immunomodulatory Functions.. Front Cell Infect Mi. 2022 Mar;12:805010-805010 WB ; Mouse. PubMed:35360110 [IF=5.293] Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199 WB ; Cattle. PubMed:35310848 [IF=4.966] Ramendu Parial. et al. Role of epigenetic m6A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR‐AKT signaling. Faseb J. 2021 May;35(5):e21465 WB ; Zebrafish. PubMed:33788967 [IF=4.872] Liqin Anet al. Benzo[a]pyrene injures BMP2-induced osteogenic differentiation of mesenchymal stem cells through AhR reducing BMPRII. Ecotoxicol Environ Saf . 2020 Oct 15;203:110930. WB ; Human. PubMed:32684523 [IF=4.667] Guo-Dong Wu. et al. Cordyceps Improves Obesity and its Related Inflammation via Modulation of Enterococcus cecorum Abundance and Bile Acid Metabolism. 2022 Mar 10 WB ; Mouse. PubMed:35282803 [IF=4.546] Ya-Nan Gao. et al. Multi-Omics Reveal Additive Cytotoxicity Effects of Aflatoxin B1 and Aflatoxin M1 toward Intestinal NCM460 Cells. TOXINS. 2022 Jun;14(6):368 WB ; Human. PubMed:10.3390/toxins14060368 [IF=4.418] Ming Hu. et al. Human Cytomegalovirus Infection Activates Glioma Activating Transcription Factor 5 via microRNA in a Stress-Induced Manner. Acs Chem Neurosci. 2021;12(20):3947–3956 WB ; Human. PubMed:34617722 [IF=4.379] Hu, Su-Qin. et al. Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-κB signaling pathway. Sci Rep-Uk. 2021 Sep;11(1):1-15 WB ; rat. PubMed:34552120 [IF=4.35] Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227 WB ; Mouse. PubMed:35563950 [IF=4.221] Haina Gao. et al. 3,5,6-trichloro-2-pyridinol intensifies the effect of chlorpyrifos on the paracrine function of Sertoli cells by preventing binding of testosterone and the androgen receptor. Toxicology. 2021 Aug;460:152883 WB ; Rat. PubMed:34352351 [IF=4.088] Yanhui Jia. et al. The deacetylation of Akt by SIRT1 inhibits inflammation in macrophages and protects against sepsis. EXP BIOL MED. ;(): WB ; Human,Mouse. PubMed:37211747 [IF=3.775] Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. WB ; Rat. PubMed:31390532 [IF=3.743] Sheng Y et al. 3-Bromopyruvate inhibits the malignant phenotype of malignantly transformed macrophages and dendritic cells induced by glioma stem cells in the glioma microenvironment via miR-449a/MCT1. Biomed Pharmacother. 2020 Jan;121:109610. WB ; Human. PubMed:31710894 [IF=3.469] Ruixi Luo. et al. Mesenchymal stem cells alleviate palmitic acid-induced endothelial-to-mesenchymal transition by suppressing endoplasmic reticulum stress. Am J Physiol-Endoc M. 2020 Nov;319(6):E961-E980 WB ; Rat. PubMed:33044844 [IF=3.405] Jianwei Lei. et al. The protective mechanism of protein kinase R to inhibit neuronal ferroptosis in cerebral injury from subarachnoid hemorrhage. BRAIN BEHAV. 2022 Jul;:e2722 WB ; Rat. PubMed:35894766 [IF=3.375] Zhang M et al. Identification of differentially expressed microRNAs and their target genes in the hippocampal tissues of Fmr1 knockout mice. Am J Transl Res . 2020 Mar 15;12(3):813-824. WB ; mouse. PubMed:32269714 [IF=3.234] Chen X et al. Oleic acid protects saturated fatty acid mediated lipotoxicity in hepatocytes and rat of non-alcoholic steatohepatitis.Life Sci. 2018 Jun 15;203:291-304. WB ; Rat&Human. PubMed:29709653
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Mouse,Rat (predicted: Human,Dog,Cow,Rabbit)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	42kDa
细胞定位	细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human ACTA1: 3-100/377
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008] Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25min muscle differentiating cells. Subcellular Location: Cytoplasm, cytoskeleton. Post-translational modifications: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. DISEASE: Nemaline myopathy 3 (NEM3) [MIM:161800]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Note=The disease is caused by mutations affecting the gene represented in this entry. Myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]: A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers and necrosis are absent. Note=The disease is caused by mutations affecting the gene represented in this entry. Myopathy, congenital, with fiber-type disproportion (CFTD) [MIM:255310]: A genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the actin family. SWISS: P68133 Gene ID: 58 Database links: Entrez Gene: 421534 Chicken Entrez Gene: 281592 Cow Entrez Gene: 58 Human Entrez Gene: 11459 Mouse Entrez Gene: 100154254 Pig Entrez Gene: 29437 Rat Omim: 102610 Human SwissProt: P68139 Chicken SwissProt: P68138 Cow SwissProt: P68133 Human SwissProt: P68134 Mouse SwissProt: P68137 Pig SwissProt: P68135 Rabbit SwissProt: P68136 Rat Unigene: 1288 Human Unigene: 214950 Mouse Unigene: 82732 Rat
产品图片	Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-Actin (bs-10966R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Actin (bs-10966R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Actin (bs-10966R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记