| 产品编号 | bs-18676R |
| 英文名称 | Marapsin1/CAPH2 Rabbit pAb |
| 中文名称 | 通道活化蛋白酶2抗体 |
| 别 名 | CAPH2; Channel activating protease 2; Marapsin; MPN; Pancreasin; Protease serine 27; PRS27_HUMAN; Prss27; Serine protease 27; UNQ1884/PRO4327. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Marapsin1/CAPH2: 151-250/290 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pancreasin is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue.[supplied by OMIM, Sep 2003] Subcellular Location: Secreted. Tissue Specificity: Expressed predominantly in the pancreas. Post-translational modifications: N-glycosylated. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: Q9BQR3 Gene ID: 83886 Database links: Entrez Gene: 83886 Human Entrez Gene: 213171 Mouse Omim: 608018 Human SwissProt: Q9BQR3 Human SwissProt: Q8BJR6 Mouse Unigene: 332878 Human Unigene: 133083 Mouse Unigene: 21284 Rat |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 40 ug
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-Marapsin1/CAPH2(bs-18676R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPH2) Polyclonal Antibody, Unconjugated (bs-18676R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
