| 产品编号 | bs-18859R |
| 英文名称 | RRP4 Rabbit pAb |
| 中文名称 | RRP4蛋白抗体 |
| 别 名 | EXOSC2; Exosome complex exonuclease RRP4; Exosome component 2; hRrp4p; OTTHUMP00000022370; OTTHUMP00000022371; OTTHUMP00000022372; OTTHUMP00000022373; OTTHUMP00000022374; p7; Ribosomal RNA processing protein 4; RRP4; Rrp4p. |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse, Macaque Monkey,Rhesus monkey,Gorilla,Opossum,Marmoset (common)) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RRP4: 51-150/293 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Rrp4 and Ski7p are components of the cytoplasmic exosome complex, and together with the RNA helicase Ski2p they are necessary for degradation of deadenylated mRNA. Rrp4 is required for the 3’ to 5’ exonuclease activity that produces the 3’ end of the 5.8S rRNA. Rrp4 also functions as a subunit of the specialized trypanosome exosome complex. Function: The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism. Subcellular Location: Cytoplasmic, Exosome and Nuclear Similarity: Belongs to the RRP1 family. SWISS: Q13868 Gene ID: 23404 Database links: Entrez Gene: 101126796 Gorilla Entrez Gene: 23404 Human Entrez Gene: 100397822 Marmoset (common) Entrez Gene: 227715 Mouse Entrez Gene: 100439017 Orangutan Entrez Gene: 595050 Xenopus tropicalis GenBank: NP_055100.2 Human Omim: 602238 Human SwissProt: Q13868 Human SwissProt: Q8VBV3 Mouse Unigene: 654643 Huma |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
