| 产品编号 | bs-18876R |
| 英文名称 | RSPO2 Rabbit pAb |
| 中文名称 | RSPO2蛋白抗体 |
| 别 名 | CRISTIN2; hRspo2; RSPO2_HUMAN; R spondin 2; R spondin 2 homolog; R-spondin 2 homolog(Xenopus laevis); R-spondin family, member 2; Roof plate-specific spondin-2. |
| 研究领域 | 细胞生物 发育生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 26 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RSPO2: 22-100/243 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008] Function: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Subunit: Interacts with WNT1. Binds heparin By similarity. Interacts with LGR4, LGR5 and LGR6. Subcellular Location: Secreted Similarity: Belongs to the R-spondin family. Contains 1 FU (furin-like) repeat. Contains 1 TSP type-1 domain. SWISS: Q6UXX9 Gene ID: 340419 Database links: Entrez Gene: 340419 Human Entrez Gene: 239405 Mouse Omim: 610575 Human SwissProt: Q6UXX9 Human SwissProt: Q8BFU0 Mouse
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| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Mouse Liver tissue lysates
Lane 5: Mouse Lung tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Large intestine tissue lysates
Lane 8: Rat Placenta tissue lysates
Lane 9: Rat Liver tissue lysates
Lane 10: Rat Lung tissue lysates
Primary: Anti-RSPO2 (bs-18876R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
