| 产品编号 | bs-18932R |
| 英文名称 | MICAL3 |
| 中文名称 | MICAL3蛋白抗体 |
| 别 名 | MICAL-3; KIAA0819; KIAA1364; MICA3_HUMAN; MICAL 3; Protein MICAL-3. |
| 研究领域 | 细胞生物 免疫学 发育生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Horse,Rabbit) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 224kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MICAL3: 21-120/2002 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MICAL3 is a 976 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Expressed ubiquitously, MICAL3 uses FAD as a cofactor to interact with Rab 1B and, via this interaction, is thought to play a role in spermatid development. MICAL3 exists as two alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 22. Chromosome 22 houses over 500 genes, some of which are involved in Phelan-McDermid syndrome, schizophrenia and Neurofibromatosis type 2. Function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process. Subunit: Interacts with RAB1B. Interacts with ERC1 and RAB8A. Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Similarity: Belongs to the Mical family. Contains 1 CH (calponin-homology) domain. Contains 1 LIM zinc-binding domain. SWISS: Q7RTP6 Gene ID: 57553 Database links: Entrez Gene: 57553 Human SwissProt: Q7RTP6 Human Unigene: 528024 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
