| 产品编号 | bs-19095R |
| 英文名称 | NECAP1 |
| 中文名称 | NECAP1蛋白抗体 |
| 别 名 | Adaptin ear binding coat associated protein 1; Adaptin ear-binding coat-associated protein 1; DKFZP566B183; MGC131900; NECAP 1; NECAP endocytosis associated 1; NECAP endocytosis-associated protein 1; NECAP-1; NECAP1; NECP1_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Cow) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NECAP1: 1-100/275 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NECAP 1 is a 275 amino acid cell membrane protein belonging to the NECAP family that localizes to the clathrin-coated vesicle membrane. NECAP 1 is encoded by a gene that maps to human chromosome 12p13.31 and contains two WXXF motifs that are used to mediate binding of accessory proteins to ear domains of various proteins such as AP-1, AP-2 and GGAs. Expressed primarily in brain and existing as two alternatively spliced isoforms, NECAP 1 colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain. Function: Involved in endocytosis. Subcellular Location: Cytoplasmic vesicle > clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane. Similarity: Belongs to the NECAP family. SWISS: Q8NC96 Gene ID: 25977 Database links: Entrez Gene: 67602 Mouse SwissProt: Q9CR95 Mouse Unigene: 52837 Cow Unigene: 288114 Mouse Unigene: 58356 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
