| 产品编号 | bs-19203R |
| 英文名称 | NPTII/Neomycin Phosphotransferase 2 |
| 中文名称 | 肺炎克雷伯菌新霉素磷酸转移酶2抗体 |
| 别 名 | Klebsiella pneumoniae Aminoglycoside 3' phosphotransferase; APH(3') II; APH(3')II; aphA; EC 2.7.1.95; Kan; Kanamycin kinase type II; Kanamycin kinase, type VIII; kanamycin resistance gene; Neo; Neomycin kanamycin phosphotransferase type II; Neomycin kanamycin phosphotransferase type VIII; Neomycin phosphotransferase II; Neomycine phosphotransferase; NPT II; NPTII; KKA2_KLEPN. |
| 研究领域 | 肿瘤 细胞生物 细菌及病毒 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Klebsiella pneumoniae) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2: 101-200/264 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. Similarity: Belongs to the aminoglycoside phosphotransferase family. SWISS: P00552 Gene ID: N/A Database links: SwissProt: P00552 Klebsiella pneumoniae |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
