| 产品编号 | bs-10794R |
| 英文名称 | Sox3 Rabbit pAb |
| 中文名称 | 转录因子Sox3抗体 |
| 别 名 | Sox-3; GHDX; Infundibular hypoplasia and hypopituitarism; MRGH; PHP; SOXB; SRY Box 3; SRY related HMG box gene 3; SRY Sex Determining Region Y Box 3; Transcription factor SOX-3; SOX3_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Sheep,Cow,Chicken) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Sox3: 101-200/446 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. Sox-3, also known as MRGH or SOXB, is implicated in mental retardation X-linked with isolated growth hormone deficiency (MRXGH) and infundibular hypoplasia and hypopituitarism. Function: SOX 3 is also known as SRY related HMG BOX gene 3. All SOX proteins have a single HMG box. Humans with mutations in SOX3 have panhypopituitarism. Conditional disruption of SOX3 in mice demonstrates that anterior pituitary development depends on SOX3 expression in the overlying neural ectoderm. Subunit: Interacts with SOX2 and FGFR1 Subcellular Location: Nuclear. DISEASE: Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123]. Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation. Similarity: Contains 1 HMG box DNA-binding domain. SWISS: P41225 Gene ID: 6658 Database links: Entrez Gene: 6658 Human Entrez Gene: 101030174 Monkey Entrez Gene: 20675 Mouse Omim: 313430 Human SwissProt: P41225 Human SwissProt: P53784 Mouse Unigene: 157429 Human Unigene: 35784 Mouse |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Fetal brain (Mouse) Lysate at 40 ug
Raji(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-Sox3 (bs-10794R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
