| 产品编号 | bs-19230R |
| 英文名称 | NFYB |
| 中文名称 | 核转录因子YB抗体 |
| 别 名 | CAAT box DNA binding protein subunit B; CBF A; CBF B; CCAAT binding transcription factor subunit A; HAP 3; HAP3; NF Y protein chain B; NF YB; NFYB; Nuclear transcription factor Y beta; Nuclear transcription factor Y subunit B; Nuclear transcription factor Y subunit beta. |
| 研究领域 | 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep,Xenopus laevis.) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NFYB: 1-100/207 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008] Function: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Subcellular Location: Nucleus. Similarity: Belongs to the NFYB/HAP3 subunit family. SWISS: P25208 Gene ID: 4801 Database links: Entrez Gene: 396205 Chicken Entrez Gene: 4801 Human Entrez Gene: 18045 Mouse Entrez Gene: 399126 Xenopus laevis Omim: 189904 Human SwissProt: P25207 Chicken SwissProt: P25208 Human SwissProt: P63139 Mouse SwissProt: 100157587 Xenopus laevis SwissProt: P25211 Xenopus laevis Unigene: 84928 Human Unigene: 245998 Mouse Unigene: 90077 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
