| 产品编号 | bs-19237R |
| 英文名称 | NHP2L2 Rabbit pAb |
| 中文名称 | X连锁脑特异性BPX蛋白抗体 |
| 别 名 | BPX; Brain specific gene BPX; Brain specific protein, X linked; Brain-specific protein; MGC26243; Nap1l2; NP1L2_HUMAN; Nucleosome assembly protein 1 like 2; Nucleosome assembly protein 1-like 2; X-linked. |
| 研究领域 | 细胞生物 神经生物学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Dog,Horse) |
| 产品应用 | WB=1:1000-3000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NHP2L2: 141-340/460 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this intronless gene is a member of the nucleosome assembly protein (NAP) family. The encoded protein represents a class of tissue-specific factors that interact with chromatin to regulate neuronal cell proliferation. [provided by RefSeq, Jan 2011] Function: Acidic protein which may be involved in interactions with other proteins or DNA. Subcellular Location: Nucleus. Similarity: Belongs to the nucleosome assembly protein (NAP) family. SWISS: Q9ULW6 Gene ID: 4674 Database links: Entrez Gene: 4674 Human Entrez Gene: 17954 Mouse Omim: 300026 Human SwissProt: Q9ULW6 Human SwissProt: P51860 Mouse Unigene: 66180 Human Unigene: 740565 Human Unigene: 388694 Mouse Unigene: 43670 Rat |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with NHP2L2 polyclonal antibody, unconjugated (bs-19237R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (bs-19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (bs-19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
