| 产品编号 | bs-19570R |
| 英文名称 | NUT |
| 中文名称 | 睾丸核蛋白NUT抗体 |
| 别 名 | C15orf55; Chromosome 15 open reading frame 55; DKFZp434O192; MGC138683; MGC138684; Nuclear protein in testis; NUT; NUT_HUMAN; Protein NUT. |
| 研究领域 | 肿瘤 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 120kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUT: 301-400/1160 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
nuclear protein in testis is a 1,132 amino acid protein that is specifically expressed in testis. Belonging to the FAM22 family, NUT shuttles between nucleus and cytoplasm via a leptomycin-sensitive pathway. It is suggested that the translocation of the NUT gene is the cause of nuclear protein in testis midline carcinomas (NMC). NMCs are highly aggressive carcinomas typically arising in midline structures in young individuals. These carcinomas are characterized by the presence of a chromosomal rearrangement of the NUT gene on chromosome 15 (15q14), which results in a chromosomal translocation most commonly involving the BRD4 gene on chromosome 19p13. Endogenous BRD-NUT fusion proteins contribute to carcinogenesis by associating with chromatin and interfering with epithelial differentiation. Subcellular Location: Cytoplasm. Nucleus. Shuttles between nucleus and cytoplasm. Tissue Specificity: Specifically expressed in testis. Post-translational modifications: Phosphorylation on Ser-1026, Ser-1029 or Ser-1031 is important for cytoplasmic export. DISEASE: Note=A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with BRD4 which produces a BRD4-NUT fusion protein. Note=A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with BRD3 which produces a BRD3-NUT fusion protein. Similarity: Belongs to the FAM22 family. SWISS: Q86Y26 Gene ID: 256646 Database links: Entrez Gene: 256646 Human Omim: 608963 Human SwissProt: Q86Y26 Human Unigene: 525769 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
