| 产品编号 | bs-19618R |
| 英文名称 | SEC61A |
| 中文名称 | 转运蛋白SEC61A抗体 |
| 别 名 | chunp6898; fb62c11; HSEC61; protein transport protein SEC61 alpha subunit; protein transport protein Sec61 subunit alpha; Protein transport protein Sec61 subunit alpha isoform 1; S61A1_HUMAN; SEC 61; SEC 61A; Sec61 alpha 1; Sec61 alpha 1 subunit; Sec61 alpha 1 subunit (S. cerevisiae); Sec61 alpha-1; SEC61; Sec61 homolog; SEC61A1; sec61aa. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Cow) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SEC61A: 181-280/476 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008] Function: Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins. Subunit: Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma. Subcellular Location: Endoplasmic reticulum membrane. Similarity: Belongs to the secY/SEC61-alpha family. SWISS: P61619 Gene ID: 29927 Database links: Entrez Gene: 29927 Human Entrez Gene: 53421 Mouse Entrez Gene: 192298 Zebrafish Omim: 609213 Human SwissProt: P61619 Human SwissProt: P61620 Mouse SwissProt: Q90ZM2 Zebrafish Unigene: 518236 Human Unigene: 28375 Mouse Unigene: 485032 Mouse Unigene: 47029 Rat Unigene: 51503 Zebrafish |
| 产品图片 |
Sample:
A431(Human) Cell Lysate at 30 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SEC61A (bs-19618R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
