| 产品编号 | bs-19853R |
| 英文名称 | RGS12 |
| 中文名称 | G蛋白信号转导调节因子12抗体 |
| 别 名 | DKFZp761K1617; Regulator of G protein signalling 12. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 156kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RGS12: 601-700/1447 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008] Function: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Isoform 5: Behaves as a cell cycle-dependent transcriptional repressor, promoting inhibition of S-phase DNA synthesis. Subunit: Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent. Subcellular Location: Nuclear Tissue Specificity: Isoform 3 is brain specific. Similarity: Contains 1 GoLoco domain. Contains 1 PDZ (DHR) domain. Contains 1 PID domain. Contains 2 RBD (Ras-binding) domains. Contains 1 RGS domain. SWISS: O14924 Gene ID: 6002 Database links: Entrez Gene: 6002 Human Entrez Gene: 71729 Mouse Omim: 602512 Human SwissProt: O14924 Human SwissProt: Q8CGE9 Mouse Unigene: 196208 Mouse Unigene: 9796 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
