| 产品编号 | bs-19964R |
| 英文名称 | TIAM2 |
| 中文名称 | T细胞淋巴瘤浸润和转移蛋白2抗体 |
| 别 名 | FLJ41865; OTTHUMP00000017472; SIF and TIAM1 like exchange factor; SIF and TIAM1-like exchange factor; STEF; T cell lymphoma invasion and metastasis 2; T lymphoma invasion and metastasis inducing protein 2; T-lymphoma invasion and metastasis-inducing protein 2; TIAM 2; TIAM-2; Tiam2; TIAM2_HUMAN. |
| 研究领域 | 肿瘤 神经生物学 信号转导 淋巴细胞 t-淋巴细胞 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 190kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TIAM2: 1601-1701/1701 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Function: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA. Subunit: Interacts with MAP1A, MAP1B, PARP1 and YWHAE. Interacts with CD44, PARD3 and MAPK8IP2 (By similarity). Subcellular Location: Cytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites. Tissue Specificity: Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis. Post-translational modifications: Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth. Similarity: Belongs to the TIAM family. Contains 1 DH (DBL-homology) domain. Contains 1 PDZ (DHR) domain. Contains 2 PH domains. Contains 1 RBD (Ras-binding) domain. SWISS: Q8IVF5 Gene ID: 26230 Database links: Entrez Gene: 26230 Human Entrez Gene: 24001 Mouse Omim: 604709 Human SwissProt: Q8IVF5 Human SwissProt: Q6ZPF3 Mouse Unigene: 586279 Human Unigene: 137134 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
