| 产品编号 | bs-21068R |
| 英文名称 | P4HA2 |
| 中文名称 | 4-羟化酶α2抗体 |
| 别 名 | 2-oxoglutarate-4-dioxygenase subunit alpha-2; 4 PH alpha 2; 4-PH alpha-2; P4HA2; P4HA2_HUMAN; procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II; Procollagen-proline; Prolyl 4 hydroxylase alpha 2 subunit; Prolyl 4 hydroxylase subunit alpha 2 [Precursor]; Prolyl 4-hydroxylase subunit alpha-2. |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P4HA2: 301-400/535 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Function: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Subcellular Location: Endoplasmic reticulum lumen. Similarity: Belongs to the P4HA family. Contains 1 Fe2OG dioxygenase domain. Contains 1 TPR repeat. SWISS: O15460 Gene ID: 8974 Database links: Entrez Gene: 8974 Human Entrez Gene: 18452 Mouse Omim: 600608 Human SwissProt: O15460 Human SwissProt: Q60716 Mouse Unigene: 519568 Human Unigene: 3705 Mouse Unigene: 79891 Rat |
| 产品图片 |
Sample:
293T(Human) Cell Lysate at 30 ug Primary: Anti-P4HA2 (bs-21068R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD 
Sample:
Hela(Human) Cell Lysate at 30 ug Primary: Anti-P4HA2 (bs-21068R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD 
Sample:
Placenta (Mouse) Lysate at 40 ug Primary: Anti- P4HA2 (bs-21068R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 58 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
