| 产品编号 | bs-12697R |
| 英文名称 | PLA2G1B |
| 中文名称 | 分泌型磷脂酶A2抗体 |
| 别 名 | Group IB phospholipase A2; Group IB secretory phospholipase A2; MGC119834; MGC119835; PA21B_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase 1B; Phospholipase A2; Phospholipase A2 group IB (pancreas); Phospholipase A2 group IB; PLA 2; PLA2; PLA2A; PLA2G1B; PPLA 2; PPLA2; sPLA2IB. |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLA2G1B: 81-148/148 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013] Function: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Subunit: Monomer or homodimer (By similarity). The inactive pro-form is a homotrimer. Subcellular Location: Secreted. Post-translational modifications: Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically. Similarity: Belongs to the phospholipase A2 family. SWISS: P04054 Gene ID: 5319 Database links: Entrez Gene: 5319 Human Entrez Gene: 18778 Mouse Omim: 172410 Human SwissProt: P04054 Human SwissProt: Q9Z0Y2 Mouse Unigene: 992 Human Unigene: 20190 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
