| 产品编号 | bs-12737R |
| 英文名称 | PLVAP Rabbit pAb |
| 中文名称 | 质膜囊泡相关蛋白抗体 |
| 别 名 | DIAR10; FELS; PV-1; PV1; gp68; PLVAP_HUMAN; PLVAP; Fenestrated endothelial-linked structure protein; Plasmalemma vesicle protein 1 (PV-1); |
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Specific References (2) | bs-12737R has been referenced in 2 publications.
[IF=17.521] Hao Sun. et al. Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner. Advanced Science. 2023 Apr;:2207020 IHC ; Human.
[IF=3.599] Li Kai. et al. A procedure in mice to obtain intact pituitary-infundibulum-hypothalamus preparations: a method to evaluate the reconstruction of hypothalamohypophyseal system. PITUITARY. 2023 Mar;:1-12 IF ; Mouse.
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| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLVAP: 301-400/442 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PLVAP (Plasmalemma Vesicle Associated Protein) is a Protein Coding gene. Diseases associated with PLVAP include Breast Benign Neoplasm and Thoracic Benign Neoplasm. GO annotations related to this gene include protein homodimerization activity. Function: Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability. Subcellular Location: Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region. Tissue Specificity: Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium. SWISS: Q9BX97 Gene ID: 83483 Database links: Entrez Gene: 83483 Human Omim: 607647 Human SwissProt: Q9BX97 Human Unigene: 107125 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Prostate tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Primary: Anti-PLVAP (bs-12737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
