| 产品编号 | bs-12766R |
| 英文名称 | VPS13D |
| 中文名称 | 液泡蛋白分选蛋白13D抗体 |
| 别 名 | KIAA0453; vacuolar protein sorting 13 homolog D (S. cerevisiae); vacuolar protein sorting 13D; Vacuolar protein sorting-associated protein 13D; VP13D_HUMAN; vps13D. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 细胞分化 淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 492kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VPS13D: 1651-1750/4388 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008] Tissue Specificity: Widely expressed. Similarity: Belongs to the VPS13 family. Contains 1 UBA domain. SWISS: Q5THJ4 Gene ID: 55187 Database links: Entrez Gene: 100050825 Horse Entrez Gene: 55187 Human Entrez Gene: 230895 Mouse Entrez Gene: 100351863 Rabbit Omim: 608877 Human SwissProt: Q5THJ4 Human Unigene: 439381 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VPS13D) Polyclonal Antibody, Unconjugated (bs-12766R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell: U-2OS cell;
Primary Antibody: Rabbit Anti-VPS13D antibody (bs-12766R) at 1:100;
Secondary Antibody: Goat Anti-Rabbit IgG-FITC at 1:200.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
