产品编号 | bs-12848R |
英文名称 | SURF5 |
中文名称 | 过量位点蛋白5抗体 |
别 名 | MED22; MED22_HUMAN; MED24; Mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22; OTTHUMP00000022480; OTTMUSP00000012503; RGD1564893; Surf 5; Surf-5; Surfeit 5; Surfeit locus protein 5. |
研究领域 | 细胞生物 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Dog,Cow,Horse,Sheep) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 22kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SURF5: 1-100/200 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Subcellular Location: Nucleus. Similarity: Belongs to the Mediator complex subunit 22 family. SWISS: Q15528 Gene ID: 6837 Database links: Entrez Gene: 417159 Chicken Entrez Gene: 6837 Human Entrez Gene: 20933 Mouse Entrez Gene: 415234 Zebrafish Omim: 185641 Human SwissProt: Q800L3 Chicken SwissProt: Q15528 Human SwissProt: Q62276 Mouse SwissProt: A5WUL3 Zebrafish Unigene: 78354 Human Unigene: 397932 Mouse Unigene: 202721 Rat Unigene: 15892 Zebrafish |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |