| 产品编号 | bs-20166R |
| 英文名称 | HCN2 |
| 中文名称 | 钾/钠超极化激活环核苷酸门控通道蛋白2抗体 |
| 别 名 | BCNG-2; BCNG2; HCN 2; Brain cyclic nucleotide gated channel 2; Brain cyclic nucleotide-gated channel 2; HAC1; Hcn2; HCN2_HUMAN; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2. |
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Specific References (1) | bs-20166R has been referenced in 1 publications.
[IF=1.468] Fei-Fei Wang. et al. Aging-induced atrial fibrosis in If current change and its effect on atrial fibrillation in dogs. ANN NONINVAS ELECTRO. 2022 Apr 11 WB ; Dog.
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| 研究领域 | 心血管 神经生物学 信号转导 通道蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Sheep,Rabbit,Cow,Dog,Rat,Mouse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HCN2: 651-750/889 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hyperpolarization-activated, cyclic nucleotide-binding channels (HCN) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides. The HCN family consists of four members (HCN1–4), each with a core transmembrane segment domain and a carboxy-terminal 120 amino-acid cyclic nucleotide-binding domain motif (1). HCN channels are expressed in the brain, heart, thalamus and testis (1). The pacemaker properties of HCN channels contribute to spontaneous rhythmic activity in the brain and heart (1). The genes encoding human HCN1 and HCN2 map to chromosomes 5 and 19p13.3, respectively (2,3). The genes encoding HCN3 and HCN4 map to chromosomes 1q21.3 and 15q24-q25, respectively (4,5). Function: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed throughout the brain. Detected at low levels in heart. Similarity: Belongs to the potassium channel HCN family. Contains 1 cyclic nucleotide-binding domain. SWISS: Q9UL51 Gene ID: 610 Database links: Entrez Gene: 610 Human Entrez Gene: 15166 Mouse Omim: 602781 Human SwissProt: Q9UL51 Human SwissProt: O88703 Mouse Unigene: 124161 Human Unigene: 12956 Mouse Unigene: 224995 Rat |
| 产品图片 |
Sample:
Lane 1: Human Jurkat cell lysates Lane 2: Human SH-SY5Y cell lysates Primary: Anti-HCN2 (bs-20166R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 110 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
